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New Insight for the Genetic Evaluation of Resistance to Ostreid Herpesvirus Infection, a Worldwide Disease, in Crassostrea gigas ArchiMer
Degremont, Lionel; Lamy, Jean-baptiste; Pepin, Jean-francois; Travers, Marie-agnes; Renault, Tristan.
The Pacific oyster, Crassostrea gigas, is the most important commercial oyster species cultivated in the world. Meanwhile, the ostreid herpesvirus 1 (OsHV-1) is one of the major pathogens affecting the Pacific oyster, and numerous mortality outbreaks related to this pathogen are now reported worldwide. To assess the genetic basis of resistance to OsHV-1 infection in spat C. gigas and to facilitate breeding programs for such a trait, if any exist, we compared the mortality of half- and full-sib families using three field methods and a controlled challenge by OsHV-1 in the laboratory. In the field, three methods were tested: (A) one family per bag; (B) one family per small soft mesh bag and all families inside one bag; (C) same as the previous methods but...
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Ano: 2015 URL: http://archimer.ifremer.fr/doc/00269/38066/36178.pdf
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Nutrition and reproduction are key parameters in the interaction process leading to Crassostrea gigas oyster summer mortality in France ArchiMer
Samain, Jean-francois; Degremont, Lionel; Soletchnik, Patrick; Ropert, Michel; Bedier, Edouard; Mazurie, Joseph; Mazurie, Joseph; Martin, Jean-louis; Moal, Jeanne; Mathieu, Michel; Pouvreau, Stephane; Lambert, Christophe; Escoubas, Jean-michel; Nicolas, Jean-louis; Le Roux, Frédérique; Renault, Tristan; Burgeot, Thierry; Bacher, Cedric; Boudry, Pierre.
Complex interactions between oyster, environment and pathogens were observed during the MOREST (2001-2005) project on summer mortality events of Crassostrea gigas oysters in France. The talk will show how nutrition level increasing reproductive intensity generates a risk for pathogen infection under synergistic effect of temperature and stress. Moreover, a genetic component is associated to specific reproductive performances and spawning behaviour. To better understand origin of such resistance or susceptibility to summer mortality, other characteristics as stress susceptibility, energy management and hemocyte activities of oysters from the two phenotypes were compared. This first comparison suggests interactions between stress, reproduction and defence...
Tipo: Text Palavras-chave: Genetic; Pathologie; Mortality; Crassostrea gigas; Pacific oyster; Reproduction; Nutrition; Physilogy.
Ano: 2006 URL: https://archimer.ifremer.fr/doc/2006/acte-3467.pdf
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Ostreid herpesvirus 1 detection and relationship with Crassostrea gigas spat mortality in France between 1998 and 2006 ArchiMer
Garcia, Celine; Thebault, Anne; Degremont, Lionel; Arzul, Isabelle; Miossec, Laurence; Robert, Maeva; Chollet, Bruno; Francois, Cyrille; Joly, Jean-pierre; Ferrand, Sylvie; Kerdudou, Nolwenn; Renault, Tristan.
Since its molecular characterisation, Ostreid herpesvirus 1 (OsHV-1) has been regularly detected in Crassostrea gigas in France. Although its pathogenicity was demonstrated on larval stages, its involvement during mortality outbreaks at the juvenile stage was highly suspected but not evidenced. To investigate mortality outbreaks, the French National Network for Surveillance and Monitoring of Mollusc Health (REPAMO) carried out two surveys in juvenile C. gigas. The first survey lasted from 1998 to 2006 and was an epidemiological inquiry occurring when oyster farmers reported mortality outbreaks. The second survey, a longitudinal one, was set up in 1998 to complete the network observations on OsHV-1. Data analysis showed a specific pattern of mortality...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00040/15110/12572.pdf
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Parental exposure to environmental concentrations of diuron leads to aneuploidy in embryos of the Pacific oyster, as evidenced by fluorescent in situ hybridization ArchiMer
Barranger, Audrey; Benabdelmouna, Abdellah; Degremont, Lionel; Burgeot, Thierry; Akcha, Farida.
Changes in normal chromosome numbers (i.e. aneuploidy) due to abnormal chromosome segregation may arise either spontaneously or as a result of chemical/radiation exposure, particularly during cell division. Coastal ecosystems are continuously subjected to various contaminants originating from urban, industrial and agricultural activities. Genotoxicity is common to several families of major environmental pollutants, including pesticides, which therefore represent a potential important environmental hazard for marine organisms. A previous study demonstrated the vertical transmission of DNA damage by subjecting oyster genitors to short-term exposure to the herbicide diuron at environmental concentrations during gametogenesis. In this paper, Fluorescent in...
Tipo: Text Palavras-chave: Aneuploidy; FISH; Diuron; Crassostrea gigas.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00238/34911/33216.pdf
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Plan de sauvegarde 2011 : synthèse de résultats 2011-2012 ArchiMer
Chavanne, Herve; Maurouard, Elise; Yonneau, Caroline; Heroin, Debora; Ledu, Christophe; Degremont, Lionel; Benabdelmouna, Abdellah.
Afin de permettre l’approvisionnement partiel des établissements ostréicoles en naissain « à survie améliorée », un protocole d’approvisionnement partiel de sauvegarde (PSI) a été mis en place en 2010 entre le CNC, les CRCs, les écloseurs et l’Ifremer afin de fournir aux ostréiculteurs du naissain présentant les meilleures chances de survie face aux mortalités estivales. Il avait ainsi été décidé que des écloseries privées produiraient du naissain triploïde « à survie améliorée » en 2010 à partir de géniteurs, femelles diploïdes et mâles tétraploïdes, sélectionnés et fournis par Ifremer. Sur la base notamment des recommandations des Assises de la Conchyliculture, l’opération a été reconduite en 2011 (PSII) et 2012 (PSIII). Suite aux productions réalisées...
Tipo: Text Palavras-chave: Mortalités; Crassostrea gigas; Huîtres creuses; Triploïde R; Sélection Génétique.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00143/25412/23563.pdf
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Plan de sauvegarde 2012, 3ème édition : synthèse des résultats 2012-2013 ArchiMer
Degremont, Lionel; Maurouard, Elise; Connes, Coralie; Ledu, Christophe; Benabdelmouna, Abdellah.
Depuis 2008 l’ostréiculture française subit des mortalités massives d'huîtres creuses, Crassostrea gigas, qui menacent les approvisionnements en naissains et, par voie de conséquence, fragilise la filière ostréicole. C’est dans ce contexte qu'a été mis en place à partir de 2010 une opération désignée sous le terme plan de sauvegarde. Cette opération sous forme contractuelle fait intervenir l'Ifremer, des écloseries privées, le Ministère en charge de l'Agriculture et le CNC. Le premier plan de sauvegarde (PS1) a été initié en 2010, suivi par un deuxième en 2011 (PS2), et un troisième en 2012 (PS3). L’objectif premier de ce plan est d’apporter une réponse temporaire au déficit de production grandissant en fournissant aux écloseries des animaux présentant une...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00229/34041/32448.pdf
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Procédures d'échantillonnage pour la conservation des espèces aquacoles dans la Cryobanque Nationale ArchiMer
Vandeputte, Marc; Labbe, Catherine; Suquet, Marc; Fauvel, Christian; Goardon, Lionel; Duclos, Delphine; Danchin-burge, Coralie; Vergnet, Alain; Rault, Paul; Degremont, Lionel; Benabdelmouna, Abdellah; Brizard, Raphael; Haffray, Pierrick.
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00098/20975/18597.pdf
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Relative importance of family, site, and field placement timing on survival, growth, and yield of hatchery-produced Pacific oyster spat (Crassostrea gigas) ArchiMer
Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Huvet, Arnaud; Moal, Jeanne; Samain, Jean-francois; Boudry, Pierre.
Summer mortality has been reported in the Pacific oyster, Crassostrea gigas, for many years in different parts of the world. The causes of this phenomenon are complex. The multidisciplinary program "MOREST", coordinated by IFREMER, was initiated to understand the causes of summer mortality of Crassostrea gigas juveniles in France and to reduce its impact on oyster production. Within this program, three successive groups of bi-parental families were bred in a hatchery in 2001 and placed in the field during summer in three sites (Ronce, Riviere d'Auray, and Baie des Veys). This paper reports the relative importance of family, site and field placement timing for three characters of major importance for oyster production: survival, growth, and yield. At the...
Tipo: Text Palavras-chave: Juvenile; Crassostrea gigas; Oysters; Yield; Growth; Survival.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-1693.pdf
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Response to divergent selection for resistance to summer mortality in Pacific oyster spat : genetic parameters and correlated responses with subsequent survival and growth ArchiMer
Boudry, Pierre; Bedier, Edouard; Ernande, Bruno; Degremont, Lionel.
The multidisciplinary program "MOREST" aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As part of this program, four successive generations of families were bred between 2001 and 2005 in our research hatchery in La Tremblade. Survival and growth were recorded in the field in three sites along the French coast (Ronce, Rivière d'Auray and Baie des Veys). The first generation (44 full-sib families nested within 17 half-sib families) was tested during summer 2001 to assess to what extent genetic variability exists for spat survival. An important genetic basis was found (estimated narrow-sense heritability among sites = 0.83 ± 0.40). In contrast, lower...
Tipo: Text Palavras-chave: Survival; Growth; Genetic parameters; Summer mortality; Selection; Genetic; Crassostrea gigas; Pacific oysters.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/acte-3458.pdf
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Response to selection for increasing resistance to the spring mortality outbreaks in Mytilus edulis occurring in France since 2014 ArchiMer
Degremont, Lionel; Maurouard, Elise; Rabiller, Manuella; Glize, Philippe.
Massive spring mortality outbreaks have been reported in Mytilus spp. in France since 2014. The main areas affected are the Pertuis Charentais Sounds and the coast of the Pays de la Loire, which cultivate mainly M. edulis, and the putative causal agents remain unknown. We report the results of the first generation of mass selection focused on survival and resistance to the spring mortality in M. edulis. Two stocks of mussels were sampled in the Pertuis Charentais Sounds in 2014; one of these went through a spring mortality outbreak and the survivors were used to produce the selected stock, while the second stock was M. edulis considered to be naïve against the putative causal agents of the mortality outbreak and was used to produce the control stock. A...
Tipo: Text Palavras-chave: Spring mortality; Survival; Mytilus edulis; Heritability; Selection.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61619/65530.pdf
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Selective breeding to improve resistance against summer mortality in the Pacific oyster Crassostrea gigas : results after 3 generations ArchiMer
Boudry, Pierre; Degremont, Lionel; Bedier, Edouard; Samain, Jean-francois.
Summer mortality of adults and juveniles has been reported in the Pacific oyster, Crassostrea gigas, for many years and in several countries. The French multidisciplinary program "Morest" aims to investigate the causes of the summer mortality in Crassostrea gigas. Within this program, we designed multi-site field experiments to assess to what extent genetic variability exists for summer mortality in French populations of C. gigas and to determine whether selective breeding could improve survival.
Tipo: Text Palavras-chave: Growth; Resistance; Selective breeding; Genetic; Crassostrea gigas; Pacific oyster; Summer mortality.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3448.pdf
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Sequence polymorphism and expression variability of Crassostrea gigas immune related genes discriminate two oyster lines contrasted in term of resistance to summer mortalities ArchiMer
Schmitt, Paulina; Santini, Adrien; Vergnes, Agnes; Degremont, Lionel; De Lorgeril, Julien.
Summer mortalities of Crassostrea gigas are a major concern in oyster aquaculture. They are the result of a complex interaction between the host, pathogens and environmental factors. Oyster genetics have been identified as an essential determinant of oyster susceptibility to summer mortalities. As the capability of oysters to circumvent diseases depends in part on their immune defenses, we aimed to analyze the gene expression and sequence polymorphism of 42 immune related genes in two oyster lines selected for their “High” (H) and “Low” (L) survival to summer mortalities. Results showed that the variability of gene expression and the sequence polymorphism acting on particular genes could enable the discrimination between H and L oyster lines. Besides, a...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26861/24979.pdf
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Several strains, one disease: experimental investigation of Vibrio aestuarianus infection parameters in the Pacific oyster, Crassostrea gigas ArchiMer
Travers, Marie-agnes; Tourbiez, Delphine; Parizadeh, Leila; Haffner, Philippe; Kozic-djellouli, Angelique; Aboubaker, Mohamed; Koken, Marcel; Degremont, Lionel; Lupo, Coralie.
This study investigated oyster infection dynamics by different strains of Vibrio aestuarianus isolated before and after the apparent re-emergence of this pathogen observed in France in 2011. We conducted experiments to compare minimal infective dose, lethal dose 50 and bacterial shedding for six V. aestuarianus strains. Whatever the strain used, mortality was induced in juvenile oysters by intramuscular injection and reached 90–100% of mortality within 5 days. Moreover, bacterial shedding was comparable among strains and reached its maximum after 20 h (≈10 EXP5 bacteria/mL/animal). Similarly, our first estimations of lethal dose 50 were comparable among strains (minimal infective dose around 0.4 × 10EXP5 bacteria/mL and LD50 around 10EXP5 bacteria/mL) by...
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Ano: 2017 URL: https://archimer.ifremer.fr/doc/00386/49768/50308.pdf
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Signature d'expression de gènes, Marqueur prédictif de capacités de survie chez l'huître Crassostrea gigas ArchiMer
Bachere, Evelyne; Degremont, Lionel; Piquemal, David; Roman, Bruno; Noguier, Florian.
Dans le contexte des mortalités massives d'huîtres Crassostrea gigas, les mécanismes moléculaires et les bases génétiques impliquées dans la capacité de survie des huîtres ont été explorées par des approches de génomique à très haut débit. Ces travaux se sont centrés dans un premier temps sur la survie à des infections par des Vibrio virulents pour lesquels des infections expérimentales étaient reproductibles et standardisées en laboratoire. Ces analyses ont conduit à la mise en évidence d'une série de gènes dont l’expression peut prédire la capacité des huîtres à survivre aux infections à vibrios (Rosa et al. 2012). Depuis 2013, les travaux ont permis la validation de la signature de survie qui a pu être corrélée avec les capacités de survie des huîtres...
Tipo: Text Palavras-chave: Huître creuse; Signature d'expression de gènes; QPCR; Biostatistique; Capacité de survie; Phénotypage; Héritabilité; Pacific oyster; Gene expression signature; QPCR; Biostatistics; Survival capacity; Phenotyping; Heritability.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00434/54551/55925.pdf
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Single or dual experimental infections with Vibrio aestuarianus and OsHV-1 in diploid and triploid Crassostrea gigas at the spat, juvenile and adult stages ArchiMer
Azema, Patrick; Travers, Marie-agnes; Benabdelmouna, Abdellah; Degremont, Lionel.
French production of the Pacific cupped oyster, Crassostrea gigas, is currently threatened by two pathogens, OsHV-1 and V. aestuarianus. While oysters selected for their higher resistance to OsHV-1 are now available for the industry, the impact of V. aestuarianus on such oysters is unknown, especially for triploids. In addition, experimental infection has used the virus or the bacteria alone, but there have been no investigations of dual exposure to these pathogens. This study is the first report of single or dual exposure in spat (Spat1 and Spat2), juvenile and adult naïve oysters. For each of the two stocks evaluated, unselected oysters and oysters selected for their higher resistance to OsHV-1 infection were tested, as well as their triploid siblings of...
Tipo: Text Palavras-chave: Vibrio aestuarianus; OsHV-1; Dual exposure; Ploidy; Selection; Crassostrea gigas.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00347/45835/45918.pdf
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Size and genotype affect resistance to mortality caused by OsHV-1 in Crassostrea gigas ArchiMer
Degremont, Lionel.
As with summer mortalities reported in France between 2001 and 2006, mortality caused by the Ostreid herpesvirus 1 (OsHV-1) in Crassostrea gigas affects mostly juveniles, although adults can also be impacted to a small extent. This could suggest that both mortalities have similar causes and that establishment of resistance, in particular to the Ostreid herpesvirus 1 (OsHV-1), depends on either the size or the age of oysters. The present study reports an investigation of both size and age using three cohorts produced during winter and three produced during summer. Each cohort contained oysters genetically selected to be resistant or susceptible to the summer mortality phenomenon, as well as unselected control oysters. Any abnormal mortality was recorded...
Tipo: Text Palavras-chave: Mortality; Crassostrea gigas; Disease resistance; Size; Age; Ostreid herpesvirus.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26799/24909.pdf
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Suivi national des mortalités au stade naissain d'un lot témoin et d'un lot à survie améliorée pour Crassostrea gigas ArchiMer
Degremont, Lionel; Maurouard, Elise; Nourry, Max; Seugnet, Jean-luc; Bedier, Edouard; Fleury, Elodie; Langlade, Aime; Pernet, Fabrice; Pouvreau, Stephane; Le Souchu, Pierrick; Normand, Julien; D'Amico, Florence; Rumebe, Myriam; Cantin, Christian; Barret, Jean; Le Gall, Patrik; Baud, Jean-pierre; Cochennec-laureau, Nathalie; Gervasoni, Erika; Pelissier, Pablo.
Ce rapport présente la comparaison des mortalités de deux lots de naissains de Crassostrea gigas dans plusieurs bassins conchylicoles et déterminer le gain de survie obtenu par la sélection pour des huîtres diploïdes
Tipo: Text Palavras-chave: Mortalités; Huître creuse; Crassostrea gigas; Sélection génétique; Naissain.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00065/17644/15180.pdf
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Summer mortality in juvenile cupped oysters : response to selection in the field and in laboratory ArchiMer
Degremont, Lionel; Bedier, Edouard; Soletchnik, Patrick; Ropert, Michel; Samain, Jean-francois; Boudry, Pierre.
The French project "Morest", coordinated by IFREMER, aims to a better understanding of the causes of summer mortalities in juvenile oysters by a multidisciplinary approach. Within this program, a first generation (G1) was bred in 2001 to assess to what extent genetic variability exists for this trait in French populations. 17 half-sib families (HSF) including 44 full-sib families (FSF) were obtained in the G1, and each FSF was reared in three sites along the French coast during the summer period. Significant differences in survival were observed among HSF, clearly indicating a genetic basis for survival. Estimation of heritability for survival was very high: 0.81  0.29. In 2002, 3 "good" HSF and 3 "bad" HSF were selected on their survival...
Tipo: Text Palavras-chave: Selection; Genetic; Mortalities; Crassostrea gigas; Oyster.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3439.pdf
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Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). I. Estimation of genetic parameters for survival and growth ArchiMer
Degremont, Lionel; Ernande, Bruno; Bedier, Edouard; Boudry, Pierre.
The multidisciplinary project "MOREST" aims to improve our understanding of causes of summer mortality in Crassostrea gigas juveniles in France and to reduce its impact on oyster production. As pail of the MOREST project, 43 full-sib families nested within 17 half-sib families were produced, planted out and tested in 3 sites during summer 2001 to assess to what extent genetic variability exists for this trait. A strong genetic basis was found for survival as narrow-sense heritability estimates ranged from 0.47 to 1.08, with higher values in sites where summer mortality was higher. Genetic correlations across sites were positive and very high for survival, indicating no genotype by environment interaction. In contrast, lower genetic variation was observed...
Tipo: Text Palavras-chave: Growth; Survival; Juveniles; Heritability; Crassostrea gigas.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2457.pdf
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Summer mortality of hatchery-produced Pacific oyster spat (Crassostrea gigas). II. Response to selection for survival and its influence on growth and yield ArchiMer
Degremont, Lionel; Bedier, Edouard; Boudry, Pierre.
Response to divergent selection for "high" and "low" survival during the summer period, from July to October, was investigated in juvenile (six-month-old) Pacific oysters, Crassostrea gigas, by producing two sets of progenies in 2002 (Generation 2) and three sets of progenies in 2003 (Generation 3). A strict between-family approach was used and resistance of these selected progenies to summer mortality was assessed in three sites along French coasts, to determine their response to selection and estimate realized heritability of the trait. A significant difference in survival was observed between the "high" and "low" selected groups at all sites for all sets of progenies, indicating a significant genetic component. High realized heritabilities for survival...
Tipo: Text Palavras-chave: Crassostrea gigas; Heritability; Juveniles; Summer mortality; Survival; Growth.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/2009/publication-6950.pdf
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